cell number measurement kit Search Results


94
Cell Signaling Technology Inc griess reagent nitrite measurement kit
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Griess Reagent Nitrite Measurement Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DIAGENODE DIAGNOSTICS highcell# chip kit c01010063
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Highcell# Chip Kit C01010063, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Zhanjiang bokang marine biological co amorphous sinps trigger vascular endothelial cell injury assay kit for endotoxin measurement in sinp suspensions
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Amorphous Sinps Trigger Vascular Endothelial Cell Injury Assay Kit For Endotoxin Measurement In Sinp Suspensions, supplied by Zhanjiang bokang marine biological co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega luciferase quantity measurement (cell titre glo® luciferase kit)
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Luciferase Quantity Measurement (Cell Titre Glo® Luciferase Kit), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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DIAGENODE DIAGNOSTICS low cell number chip kit protein a × 48
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Low Cell Number Chip Kit Protein A × 48, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega remaining cell numbers (celltiter-glo kit)
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Remaining Cell Numbers (Celltiter Glo Kit), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson white blood cell counting kit leucocount
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
White Blood Cell Counting Kit Leucocount, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega cell viability measuring kit luciferase-based celltiter-glo
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Cell Viability Measuring Kit Luciferase Based Celltiter Glo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega cell viability measuring kit methanethiosulfonate [mts]–based celltiter-one
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Cell Viability Measuring Kit Methanethiosulfonate [Mts]–Based Celltiter One, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Oculyze GmbH yeast cell numbers determination kit
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Yeast Cell Numbers Determination Kit, supplied by Oculyze GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega cell viability assay measuring atp levels in cell lysates with a commercially available kit celltiter-glo
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Cell Viability Assay Measuring Atp Levels In Cell Lysates With A Commercially Available Kit Celltiter Glo, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega atp measurement kit chemical bactiter-glo microbial cell viability assay
Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the <t>Griess</t> <t>reagent</t> (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.
Atp Measurement Kit Chemical Bactiter Glo Microbial Cell Viability Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the Griess reagent (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.

Journal: Redox Biology

Article Title: Procyanidin B2 mitigates endothelial endoplasmic reticulum stress through a PPARδ-Dependent mechanism

doi: 10.1016/j.redox.2020.101728

Figure Lengend Snippet: Inhibition of PPARδ abrogated the effects of PCB2 on NO and ROS production. ECs were pretreated with GSK0660 (1 μM) for 1 h and then treated with PCB2 (10 μM) for 12 h before exposure to HG (30 mM) for 24 h. (A) Confocal microscopic detection of superoxide with DHE. (B) The mean fluorescence intensity was evaluated. Scale bar: 25 μm (n = 3). (C) Production of ROS was measured with L-012 chemiluminescence (n = 5). (D) Summerized levels of intracellular NO production in ECs detected as DAF FM-DA signals before (F0) and after (F1) the addition of A23187 (n = 5). (E) Nitrite levels in EC supernatants was measured by using the Griess reagent (n = 4). All data were expressed as mean ± SEM. * P < 0.05, ** P < 0.01 vs. vehicle; # P < 0.05, ## P < 0.01 vs. HG; † P < 0.05, †† P < 0.01 vs. PCB2+HG.

Article Snippet: Antibodies against adenosine monophosphate-activated protein kinase (AMPK), phospho-AMPK (Thr 172 ), activating transcription factor 4 (ATF4), ATF6, GRP78, protein-disulfide isomerase (PDI), IRE1α and Griess reagent nitrite measurement kit were from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Inhibition, Fluorescence